Speaking in Moscow on Sunday, Navalny declared "an election without us is not an election," ahead of submitting his nomination to the Central Electoral Commission, which will rule whether or not he can run.
Russian authorities have deemed the 41-year-old ineligible to run due to a criminal conviction, saying "only a miracle" would help him get registered.
Critics of President Vladimir Putin believe the conviction to be politically motivated.
Thousands of Russian's backing the potential opposition candidate met in 20 cities from the Pacific port of Vladivostok to Saint Petersburg in the northwest to nominate him as their choice in the presence of electoral officials, in an apparent bid to boost his chances of contesting the 18 March vote.
According to his campaign team, more than 15,000 people endorsed him nationwide and under Russian law an independent candidate needs 500 votes to be registered with election authorities.
This comes as President Putin announced earlier this month that he will seek a fourth presidential term, which would extend his rule until 2024.
This would make him the longest-serving Russian leader since dictator Joseph Stalin.