The venue contract for Dieudonné's performance was cancelled by a public-funded Avignon tourism company due to the "opacity" of the contents of his show.
A court in Nîmes, near Avignon, on Tuesday refused an urgent appeal by Dieudonné's lawyer Jacques Verdier after an Avignon judge ruled in favour of the company.
The lease was cancelled in December, four months after it was signed.
While tickets for the show were sold as Dieudonné Tour, the performance was listed on his website as The Filthy Beast, a lawyer for the company told the AFP news agency.
A clause in the lease made it mandatory for Dieudonné's production company to inform the venue's manager of the show's content.
His lawyer says he is "appalled" and will make a claim in court for compensation.
His arrest was one of dozens of cases opened for “condoning terrorism”.
Dieudonné has also come under fire for his trademark “quenelle” hand gesture that looks like an inverted Nazi salute, which he insists is merely anti-establishment and not anti-Semitic as has been charged.