Tehran on Friday denied the United States had downed one of its drones, saying all Iranian aircraft were accounted for and jesting Washington may have accidentally hit their own machine.
The latest incident in the strategic Strait of Hormuz comes amid soaring tensions between the two foes, with Washington already reeling from Iran shooting down one of its drones last month.
US President Donald Trump said an American naval vessel downed an Iranian drone on Thursday that threatened the ship as it was entering the Strait -- a claim vehemently denied by Tehran.
Iran's armed forces dismissed Trump's assertion as "baseless and delusional".
"All of (Iran's) drones... have safely returned to their bases," said armed forces spokesman Brigadier General Abolfazl Shekarchi.
"There have been no reports of a confrontation with the American USS Boxer" naval vessel, he added, quoted by Tasnim.
Trump said Thursday the USS Boxer "took defensive action" against an Iranian aircraft as it was "threatening the safety" of the vessel and its crew.
The Pentagon said the ship "was in international waters" when a drone approached.
"The ship took defensive action against the UAS to ensure the safety of the ship and its crew," Pentagon spokesman Jonathan Hoffman said.
But Iran's deputy foreign minister Abbas Araghchi said such an incident had never taken place, saying the Islamic republic had not lost any aircraft.
"I am worried that USS Boxer has shot down their own (drone) by mistake!" Abbas Araghchi tweeted Friday.
- Iran detains tanker -
The confrontation comes after Tehran shot down an American surveillance drone in June it said was flying in its airspace, a claim denied by the United States.
Trump said he called off retaliatory strikes at the last minute following the incident.
Iran has in turn refuted US accusations that it was behind a series of recent tanker attacks off the United Arab Emirates coast and the Gulf of Oman.
The tanker troubles further intensified on July 4, when Gibraltar detained an Iranian vessel with the help of British Royal Marines.
US officials believed the tanker was destined for Syria to deliver oil, in violation of separate sets of European Union and US sanctions.
Iran furiously branded the seizure "piracy", and one week later, London said Iranian boats menaced a British tanker in the Gulf before they were driven off by a Royal Navy frigate.
That led to US calls for an international flotilla to escort hydrocarbon-laden vessels from Gulf oil fields through the Hormuz Strait.
The incidents have raised fears of a regional conflict involving the US and its allies in the Gulf region, through which nearly a third of the world's oil is transported.
The top commander of Iran's Revolutionary Guards' said they were not seeking to "initiate a war" but would respond to hostilities.
"If enemies make a mistake in calculation, our defensive strategy and all our capacities will change to offensive," said General Hossein Salami, quoted by the force's Sepahnews website.
His comments came after the Guards said Thursday they had seized a "foreign tanker" believed to be the Panamanian-flagged vessel Riah and its crew.
Iran's Revolutionary Guards accuse the ship of smuggling Iranian fuel.
- 'Psychological pressure' -
US Central Command chief Kenneth McKenzie pledged Thursday to work "aggressively" with partners to ensure freedom of navigation in Gulf waters.
But the Guards' deputy chief said the United States will soon realise "it is not in its interest at all to stay in the region".
US forces are under mounting "psychological pressure" in the Gulf, Brigadier-General Ali Fadavi was quoted by Tasnim as saying.
The drone incident comes amid a threatened breakdown of the 2015 Iran nuclear deal, with Washington steadily ratcheting up economically debilitating sanctions on Tehran a year after unilaterally quitting the deal.
Earlier this month, Tehran purposely surpassed the deal's caps on uranium enrichment, aiming to pressure the remaining parties to make good on their promises to support Iran economically.
Iran has repeatedly threatened to close the Strait of Hormuz if Tehran's oil exports are blocked through sanctions.
Last month Oil Minister Bijan Namdar Zanganeh said Iran was keeping up sales through "unconventional" means to circumvent the US measures.